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OBJECTIVE To establish a method for simultaneous determination of 8 aminoglycoside antibiotics (AGs) including kanamycin, gentamycin C1, gentamycin C2, gentamycin C1a, tobramycin, neomycin, isepamicin and etimicin by LC-MS/MS with instant library searching. METHODS Eight AGs were separated by Shiseido PC HILIC (2.0 mm×100 mm, 3 μm) column, with 0.5% formic acid aqueous solution-0.1% formic acid acetonitrile solution as mobile phase and 0.4 mL·min-1 of flow rate,and procedure of the gradient elution was performed. The screening method of 8 AGs was established by the scanning mode of multiple reaction monitoring (MRM). Typical mass spectrograms of 8 AGs were collected by collision energy expansion (CES) function and enhanced ion (EPI) scanning mode, and added them to the already established mass spectralibrary of antibiotics searching. RESULTS The established method has the advantages of short analysis time, strong specificity and high sensitivity. The screening and qualitative analysis of 8 AGs can be realized within 10 min. Three batches of the INSA insulin product were detected by this method, and kanamycin that was less than the detection limit of 0.05 ng·mg-1 were not detected in above samples. CONCLUSION In this study, the detection method of 8 AGs established by LC-MS/MS with instant library searching can quickly screen and accurately determine trace AGs.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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