PDF
Full
Outline
OBJECTIVE To explore the mechanism of Shengqing Huazhuo (SQHZ) prescription in regulating simple obesity rats with spleen deficiency and dampness obstruction. METHODS The rat model of simple obesity with spleen deficiency and dampness obstruction was established, and the effects of SQHZ prescription on body weight, liver histomorphology and expression level of blood lipid metabolism in obesity rats were comprehensively evaluated. Ultra-high performance liquid chromatography-Q/Exactive mass spectrometry (UHPLC-QE-MS) was used to identify the blood components of SQHZ prescription, and the potential action pathways and targets were predicted by the network pharmacological platform. Non-targeted metabonomics was used to analyze the metabolomics of rat serum. The abundance of intestinal flora in rat faeces was detected by 16S rDNA amplicon sequencing technology. Finally, a multi-scale and multi-dimensional network was constructed by summarizing the KEGG pathways of the three, which can be used for overall visualization and deep analysis. RESULTS SQHZ prescription significantly reduced the body weight of obesity rats, and improved blood lipid levels and liver fat accumulation. Twenty-seven blood components of SQHZ prescription were identified by UHPLC-QE-MS technology. Network pharmacological analysis revealed the pathways related to metabolism of SQHZ prescription, mainly involving steroid hormone biosynthesis and ovarian steroidogenesis. Serum metabolomics analysis obtained 10 key differential metabolites, which involved metabolic pathways such as biosynthesis of unsaturated fatty acids and tryptophan metabolism. Intestinal microbiota sequencing results showed that SQHZ prescription could regulate the composition and improve the structure of intestinal microbiota in obesity rats, and the metabolic pathways involved are the biosynthesis of steroid hormones and unsaturated fatty acids. CONCLUSION SQHZ prescription not only shows significant therapeutic effects in treating simple obesity rats with spleen deficiency and dampness obstruction, but also has the ability to regulate serum metabolism and intestinal microbial community structure.
PDF
101
50
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
