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OBJECTIVE To establish the HPLC fingerprints, determine the contents of chlorogenic acid, liquiritin, lobetyolin, liquiritigenin, glycyrrhetinic acid, atractylenolide Ⅲ, atractylenolide Ⅱ, atractylenolide Ⅰ, 10-gingerol in Lizhong Pills, and lay the foundation for its quality control. METHODS A Eclipse Plus C18 column was used with acetonitrile-0.1% aqueous phosphoric acid as the mobile phase in gradient elution at a flow rate of 0.8 mL·min-1. The detection wavelengths were set at 215 nm and 254 nm, and the column temperature was maintained at 30 ℃. Through similarity evaluation, combined with chemical pattern recognition, the fingerprints of 21 batches of Li Zhong Pills were evaluated and analyzed, and 9 index components were quantitatively determined. RESULTS The fingerprints of 21 batches of Lizhong Pills were established, with 29 peaks identified, the similarity of each batch was greater than 0.9, and 21 batches of samples from 3 manufacturers could be clustered into 3 categories, with 16 compounds differing between groups. Nine components showed good linear relationships within their respective linear ranges (r≥0.998 7), the average recoveries ranged from 95.34% to 104.41%, and the RSDs ranged from 0.95% to 2.95%. CONCLUSION The fingerprint and quantitative determination method of Lizhong Pills is simple, accurate and reproducible, and can be used for the evaluation of the overall quality of Lizhong Pills.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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