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OBJECTIVE To idenntify the whole chemical components of different extracts of alangium chinense root and fibrous root using ultra high performance liquid chromatography-mass spectrometry(UPLC-MS/MS), and screen the index components of different medicinal parts”. METHODS UPLC-MS/MS technology was used to analyze the total components of alcoholic extract (ALE), water extract (ALW) of Alangium chinense root, alcoholic extract (AFE) and water extract (AFW) of Alangium chinense fibrous root. Principal component analysis (PCA), orthogonal partial least squares-discriminant analysis (OPLS-DA) and other multivariate statistical analysis were used to screen and analyze the chemical components with significant differences in different medicinal parts of Alangium chinense. RESULTS A total of 406 active chemical components were identified from the extract of Alangium chinense. The mass spectrometry cracking rules of phenolic acids, flavonoids, alkaloids and terpenoids were summarized and analyzed in different medicinal parts. Twenty Index components such as fraxin, loganic acid and benzoyleneurea were screened from different medicinal parts. CONCLUSION There were obvious differences in the components of different medicinal parts and different extracts of Alangium chinense. Phenolic acids, flavonoids and alkaloids are the main compounds, and they were mainly concentrated in flavonoid biosynthesis pathway. This study provides a scientific basis for clarifying the pharmacodynamic material basis and quality differences of different parts of Alangium chinense, and lays a foundation for rapid identification, quality control and further development and utilization of Alangium chinense.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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