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OBJECTIVE To establish the fingerprints of 50 batches of Arnebiae Radix and the methods for determination of the contents of six components. METHODS A high performance liquid chromatographic (HPLC) method was used for the determination of 50 batches of Arnebiae Radix on an Agilent 5 TC-C18(2) column, with acetonitrile-0.05% formic acid aqueous solution as the mobile phase, gradient elution at a flow rate of 1 mL·min-1, detection wavelength of 275 nm, and column temperature of 30 ℃. The contents of six naphthoquinone components, i.e., alkannin, acetylshikonin, deoxyshikonin, β-acetoxyisovalerylshikonin, isobutyryl alkannin, and β,β'-dimethylacrylalkannin were determined. The fingerprints of the herbs were established by HPLC fingerprinting combined with the “Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System (Version 2012A)” to evaluate the similarity and confirm the common peaks. RESULTS The methodological validation results showed that the peak areas of the six components had good linear relationships in the corresponding concentration ranges, and the contents of the six components ranged from 0.006%-0.237%, 0.009%-1.759%, 0.001%-0.129%, 0.002%-0.625%, 0.011%-0.609%, and 0.016%-1.712%, respectively. The HPLC fingerprints of Arnebiae Radix were established, and a total of nine common peaks were calibrated. The similarity fitting was performed by using the TCM fingerprinting software, and it was found that the similarity of the 50 batches of Arnebiae Radix varied greatly. CONCLUSION The HPLC fingerprint of Arnebiae Radix established in this study and the method of simultaneous determination of the contents of six components are simple, stable, accurate, reliable, and reproducible, which can provide a basis for the quality study of Arnebiae Radix.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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