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OBJECTIVE To establish the fingerprint and content determination method of Impatientis Pritzelii Rhizoma to improve the quality control method of Tujia ethnomedicinal herb Impatientis Pritzelii Rhizoma. METHODS The fingerprint was established by UPLC with ACQUITY UPLC BEH C18 column. Phlorizin content assay was performed by HPLC with Eclipse XDB-C18 column. The mobile phases for both methods were composed of acetonitrile and 0.1% formic acid with different gradient elution procedures. The detection wavelength was set at 283 nm. The comparative study was carried out between Impatientis Pritzelii Rhizoma and its adulterants (such as the roots and rhizomes of Impatiens pinfanensis, I. uliginosa, I. siculifer and I. noli-tangere). RESULTS Nine main chromatographic peaks in the fingerprint could be served as the characteristic peaks of Impatientis Pritzelii Rhizoma, four of which were corresponding to catechin, epicatechin, scopolamine and phlorizin, respectively. The established fingerprint could reflect the distribution of the chemical components of Impatientis Pritzelii Rhizoma, which was distinguished from four adulterants. Phlorizin was found and determined in Impatientis Pritzelii Rhizoma for the first time. The contents in 16 batches of Impatientis Pritzelii Rhizoma ranged from 0.007% to 0.085%, while no phloridin was found in the adulterants. CONCLUSION The established methods for the fingerprint and phlorizin content determination of Impatientis Pritzelii Rhizoma are specific, accurate and effective, which could be used for its quality control.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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