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OBJECTIVE To establish the HPLC fingerprint and multi-component quantitative determination method of Glehniae Radix, and study the dispersion in quality of Glehniae Radix of different regions and residual root bark ratio by chemical pattern recognition. METHODS HPLC method was used to establish the fingerprint and determine the contents of seven active components. The chromatographic column was ShimNex CS C18(4.6 mm×250 mm, 5 μm), the mobile phase was methanol-0.4% phosphoric acid water, gradient elution was conducted at a volume flow rate of 1.0 mL·min-1, the column temperature was maintained at 30 ℃, and the detection wavelength was set at 204 and 248 nm. Fingerprint similarity was used to evaluate different regions of Glehniae Radix. Cluster analysis, principal component analysis and discriminant analysis were used to analyze the quality and the differential components of Glehniae Radix of different residual root bark ratio. RESULTS A total of 18 common peaks were found in the fingerprints of 60 batches of Glehniae Radix fingerprints from different regions, and seven peaks were identified, including facarindiol, panaxynol, xanthotoxin, bergapten, imperatorin, isocorallol and isoimperatorin. The similarity of 60 batches of samples was 0.814-0.996. The multi-component contents of the 190 batches of Glehniae Radix were divided into two groups by cluster analysis, and the samples with residual root bark ratio ≤1/3 and >1/3 were significantly distinguished. The results of PCA and HCA were consistent. The different quality markers were panaxynol and facalindiol. CONCLUSION The established HPLC fingerprint and multi-component content determination method are simple, stable, and reliable, which can provide a basis for the quality control, overall evaluation and standardized processing methods of Glehniae Radix.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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