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OBJECTIVE To establish an UHPLC-MS/MS method for the determination of six alkaloids in Tianma capsule. METHODS Phenomenex Kinetex C18 (2.1 mm×100 mm, 2.6 μm) column was used for separation. The mobile phase consisted of methanol and 0.1% formic acid (containing 2.5 mmol·L-1 ammonium acetate) solution (gradient elution). The flow rate was 0.3 mL·min-1 and the column temperature was maintained at 35 ℃. Electrospray positive ion source was used for mass spectrometry detection by multiple reaction monitoring mode. RESULTS The liner range of calibration curve was 79.38-3 969 ng·mL-1 (r=0.999 9), 18.60-929.8 ng·mL-1 (r=0.999 8), 38.20-1 910 ng·mL-1 (r=0.999 9), 1.264-252.8 ng·mL-1(r=0.999 3), 1.196-239.2 ng·mL-1(r=1.000 0) and 1.268-253.6 ng·mL-1(r=1.000 0)for benzoylmesaconine, benzoylaconine, benzoylhypaconine, mesaconitine, hypaconitine and aconitine. The RSD of repeatability test (content) was 0.5%-3.2%. The average recoveries was in the range of 98.3%-105.3%. RSD was 1.0%-3.4%. CONCLUSION The method has good sprcificity, sensitivity and accuracy, and it is suitable for the analysis of six alkaloids in Tianma capsule.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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