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Establishment of UPLC Fingerprint of Different Parts of Gossampim Flos and Study on Spectrum Effect Relationship
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Xintong YU, Qian CAO, Lanlan ZHANG, Kaidong WANG, Zaoshu CHEN, Ling PAN, Zheng ZHANG*
Chinese Pharmaceutical Journal | 2024, 59(22) : 2132 - 2139
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Chinese Pharmaceutical Journal | 2024, 59(22): 2132-2139
Establishment of UPLC Fingerprint of Different Parts of Gossampim Flos and Study on Spectrum Effect Relationship
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Xintong YU, Qian CAO, Lanlan ZHANG, Kaidong WANG, Zaoshu CHEN, Ling PAN, Zheng ZHANG*
Affiliations
  • Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule, Guangdong Yi Fang Pharmaceutical Co., Ltd., Foshan 528244, China
Published: 2024-11-22 doi: 10.11669/cpj.2024.22.005
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OBJECTIVE To determine the antioxidant activities of Gossampim Flos waterextracts,establish UPLC fingerprints and study the spectral effect relationship. METHODS Based on the scavenging rates of 2, 2-diazo-di (3-ethyl-benzothiazolin-6-sulfonic acid) diamiammonium salt (ABTS) and 1, 1-diphenyl-2-picrohydrazyl radical (DPPH), the fingerprint of different parts (petal, calyx and stamen) of 12 batches of Gossampim Flos were established by UPLC method, and similarity analysis were carried out. Grey correlation analysis (GRA), Spearman analysis, stepwise regression analysis and partial least squares regression(PLSR) analysis were used to study the relationship between antioxidant spectrum effect. RESULTS Eight chromatographic peaks were identified by fingerprint identification of water extracts from different parts of 12 batches of Gossampim Flos. Mangiferin, quercetin, rutin, vitexin and other compounds were the main active components of Gossampim Flos and its different parts for antioxidant activities. CONCLUSION The antioxidant activity of Gossampim Flos and its different parts are the result of the combined effect of multiple components. This study can provide a comprehensive reference for the basis and quality control of antioxidant active substances of Gossampim Flos and its different parts.

Gossampim Flos  /  antioxidant  /  fingerprint  /  2, 2-diazo-di (3-ethyl-benzothiazol-6-sulfonic acid) diamiammonium salt  /  1, 1-diphenyl-2-picrohydrazine radical  /  spectral effect relationship
Xintong YU, Qian CAO, Lanlan ZHANG, Kaidong WANG, Zaoshu CHEN, Ling PAN, Zheng ZHANG. Establishment of UPLC Fingerprint of Different Parts of Gossampim Flos and Study on Spectrum Effect Relationship[J]. Chinese Pharmaceutical Journal, 2024 , 59 (22) : 2132 -2139 . DOI: 10.11669/cpj.2024.22.005
Year 2024 volume 59 Issue 22
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doi: 10.11669/cpj.2024.22.005
  • Receive Date:2024-05-09
  • Online Date:2025-11-16
  • Published:2024-11-22
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  • Received:2024-05-09
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    Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Formula Granule, Guangdong Yi Fang Pharmaceutical Co., Ltd., Foshan 528244, China
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表12种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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