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Optimization of Preparing Technology of Total Saponins form Schizocapsa plantaginea Hance and its Quality Control Method
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Hao LI1, Jinhua LI1, Yuxue MO1, Meixian LV1, Xinyi GUO1, Ziying LIANG1, Yanxue WANG1, Buming LIU2, Gang LIANG1, 2, 3, *, Jiantong YUAN2, *
Chinese Pharmaceutical Journal | 2024, 59(23) : 2278 - 2287
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Chinese Pharmaceutical Journal | 2024, 59(23): 2278-2287
Optimization of Preparing Technology of Total Saponins form Schizocapsa plantaginea Hance and its Quality Control Method
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Hao LI1, Jinhua LI1, Yuxue MO1, Meixian LV1, Xinyi GUO1, Ziying LIANG1, Yanxue WANG1, Buming LIU2, Gang LIANG1, 2, 3, *, Jiantong YUAN2, *
Affiliations
  • 1 College of Pharmacy, Guangxi Medical University, Nanning 530021, China
  • 2 Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards, Nanning 530015, China
  • 3 State Key Laboratory of Targeted Oncology, Guangxi Medical University, Nanning 530021, China
  • 4 Guangxi Key Laboratory of Bioactive Molecules Research and Evaluation, Nanning 530021, China
Published: 2024-06-19 doi: 10.11669/cpj.2024.23.010
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OBJECTIVE To establish a method for preparing total saponins from Schizocapsa plantaginea Hance and establish a corresponding quality control method. METHODS The total saponins were extracted from the Schizocapsa plantaginea Hance tubers using a water bath reflux method, followed by purification of the crude extract through solvent extraction and macroporous resin. Subsequently, the purified product of total saponins of Schizocapsa plantaginea Hance(SSPHs) was prepared, and its quality was assessed using thin-layer chromatography, UV-visible spectrophotometry, and ultra-high performance liquid chromatography-mass spectrometry(UHPLC-MS) combined method. RESULTS The developed preparation method resulted in a yield of total saponin products from the Schizocapsa plantaginea Hance tuber ranging from 0.29% to 2.81%, with a purity from 57.30% to 77.02%. After detection, saponin substances such as Schizocapsa Plantaginea Hance Ⅰ(SSPH Ⅰ) and Schizocapsa plantaginea Hance Ⅱ(SSPH Ⅱ) were found in the total saponin products. The content of SSPH Ⅰ was 10.77-85.60 mg·g-1 and the content of SSPH Ⅱ was 0.19-4.75 mg·g-1. CONCLUSION The established preparation method is simple, efficient, and environmentally friendly, with high product quality. The established qualitative and quantitative detection method can effectively reflect the quality of SSPHs and provide method reference for the quality control of SSPHs.

Schizocapsa plantaginea Hance  /  total saponin  /  UHPLC-MS/MS  /  quality control
Hao LI, Jinhua LI, Yuxue MO, Meixian LV, Xinyi GUO, Ziying LIANG, Yanxue WANG, Buming LIU, Gang LIANG, Jiantong YUAN. Optimization of Preparing Technology of Total Saponins form Schizocapsa plantaginea Hance and its Quality Control Method[J]. Chinese Pharmaceutical Journal, 2024 , 59 (23) : 2278 -2287 . DOI: 10.11669/cpj.2024.23.010
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Year 2024 volume 59 Issue 23
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Article Info
doi: 10.11669/cpj.2024.23.010
  • Receive Date：2024-06-12
  • Online Date：2025-11-13
  • Published：2024-06-19
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  • Received：2024-06-12
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Affiliations
    1 College of Pharmacy, Guangxi Medical University, Nanning 530021, China
    2 Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards, Nanning 530015, China
    3 State Key Laboratory of Targeted Oncology, Guangxi Medical University, Nanning 530021, China
    4 Guangxi Key Laboratory of Bioactive Molecules Research and Evaluation, Nanning 530021, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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