PDF
Full
Outline
OBJECTIVE To investigate the effect of tea polyphenols (TP) on aspirin-induced injury of human gastric epithelial cells GES-1 and its molecular mechanism. METHODS GES-1 cells were treated with aspirin (19.27 mmol·L-1) and different concentrations of tea polyphenols (50, 100, 200 μmol·L-1) for 24 h. GES-1 cells were randomly divided into control group, aspirin group(19.27 mmol·L-1), aspirin 19.27 mmol·L-1+TP 50 μmol·L-1 group, aspirin 19.27 mmol·L-1+TP 100 μmol·L-1 group, aspirin 19.27 mmol·L-1+TP 200 μmol·L-1 group. Methyl thiazolyl tetrazolium (MTT) was used to detect the survival rate of GES-1 cells. Flow cytometry and TUNEL assay were used to detect apoptosis of GES-1 cells. Dichlorofluorescein diacetate (DCFH-DA) fluorescent probe was used to detect the levels of reactive oxygen species (ROS) of GES-1 cells. Corresponding kits were used to detect the levels of superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) of GES-1 cells. Western blot was used to detect the expression levels of B-lymphocytoma-2 (Bcl-2), B-lymphocytoma-2-associated X protein (Bax), nuclear factor E2 related factor 2 (Nrf2) and heme monooxygenase 1 (HO-1) of GES-1 cells. RESULTS Compared with the control group, the apoptosis rate, apoptosis index, ROS, MDA levels, nuclear Nrf2/ cytoplasmic Nrf2 ratio, Bax, and HO-1 protein expression levels of GES-1 cells in 19.27 mmol·L-1 aspirin group were significantly increased, while the cell survival rate, Bcl-2 protein expression level, SOD and CAT levels were significantly decreased (P<0.05). Compared with 19.27 mmol·L-1 aspirin group, the apoptosis rate, apoptosis index, ROS, MDA levels and Bax protein expression levels in aspirin 19.27 mmol·L-1+TP (50, 100, 200 μmol·L-1)groups were significantly decreased, while the cell survival rate, nuclear Nrf2/cytoplasmic Nrf2 ratio, Bcl-2, Nrf2, HO-1 protein expression levels, SOD and CAT levels were significantly increased (P<0.05).CONCLUSION Tea polyphenols can alleviate aspirin-induced GES-1 cell damage, and the mechanism may be related to the activation of Nrf2/HO-1 antioxidant pathway and the inhibition of mitochondrial pathway mediated apoptosis.
PDF
126
60
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
