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OBJECTIVE To establish UPLC fingerprint of Aster tataricus cv.Qiziwan, identify the compounds of common peaks by UPLC-Q-TOF-MS technology, determine antioxidant activity of each batch and study the relationship between effects of antioxidant and spectrum of Aster tataricus cv.Qiziwan. METHODS The fingerprints of 22 batches of Aster tataricus cv.Qiziwan were drawn and evaluated by the the Similarity Evaluation System of TCM Chromatographic Fingerprint (2012 edition), and the common peaks were calibrated. The antioxidant activity of Aster tataricus cv.Qiziwan was investigated by using the free radical scavenging rate of 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) and 2, 2'-azinobis- (3-ethylbenzthiazoline-6-sulphonate) (ABTS) free radical scavenging rate as the antioxidant index. The spectral effect relationship between chemical constituents and antioxidant activity of Aster tataricus cv.Qiziwan was analyzed by grey correlation degree and partial least squares regression. RESULTS The fingerprints of 22 batches of Aster tataricus cv.Qiziwan from different batchs were established, and the similarity was 0.892-0.995. Cluster analysis can distinguish rhizome with mother root, root and rhizome. Seventeen common peaks were identified by comparison of reference materials and UPLC-MS analysis. The DPPH free radical and ABTS free radical scavenging experiments showed that 22 batches of Aster tataricus cv.Qiziwan had antioxidant capacity, and the grey correlation analysis showed that shionone had the highest correlation degree. Combined with partial least squares regression analysis, it was preliminarily confirmed that rutin, ferulic acid, isochlorogenic acid A, astin A, quercetin, perillen and shionone were the main components of Aster tataricus cv.Qiziwan with antioxidant activity. CONCLUSION In this study, the fingerprint and antioxidant activity spectrum effect relationship of Aster tataricus cv. Qiziwan are successfully established, which can provide reference for the pharmacodynamic material basis research and quality control of Aster tataricus cv.Qiziwan.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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