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OBJECTIVE To optimized the extraction process of Ganoderma lucidum crude polysaccharide using Ganoderma lucidum fruiting body as experimental material, and discuss its antioxidant activity. METHODS In this study, the extraction conditions of crude polysaccharide from Ganoderma lucidum were optimized by single factor experiment and response surface methodology. The polysaccharide was extracted by hot water extraction method, protein was removed by Sevag method, and purified by anion exchange column chromatography and gel column chromatography. Its structure was preliminarily determined by UV spectrum, FT-IR spectrum and atomic force microscope. The antioxidant activity of Ganoderma lucidum polysaccharide was evaluated by detecting DPPH radical, ABTS radical, hydroxyl radical and superoxide anion scavenging ability. RESULTS The results showed that the best extraction conditions of Ganoderma lucidum crude polysaccharide was extraction temperature 92.4 ℃, extraction time 5 h 5 min, liquid-solid ratio 21∶1, and the yield was 2.967±0.228%. Ganoderma lucidum polysaccharide obtained by extraction and purification conform to the structural characteristics of polysaccharide, and did not contain nucleic acids and proteins. Atomic force microscopy showed that most of them presented a chain like conformation. Ganoderma lucidum polysaccharide can significantly scavenge DPPH radical, ABTS radical, hydroxyl radical and superoxide anion. CONCLUSION Ganoderma lucidum polysaccharide has good antioxidant ability, this study provides a basis for further development and application of Ganoderma lucidum.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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