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OBJECTIVE To identify Bambusae Caulis In Taenias and its adulterants using DNA barcoding,ensure the safety andefficacy of clinicalapplications and provide a referencefor the development and quality evaluation of Bambusae Caulis In Taenias-containing drugs. METHODS By using accurately original plants bamboo samples as a reference, and based on the screening of universal barcodes, variable regions or loci within the chloroplast genome were identified to design genus-specific DNA barcodes for the molecular identification of commercial Bambusae Caulis In Taenias. RESULTS ITS2 region effectively identified Phyllostachys edulis and allowed for the preliminary identification of other species at the genus level. Additionally, 17 genus-specific DNA barcodes were designed, enabling the precise identification of Phyllostachys nigra var. henonis, Bambusa beecheyana var. Pubescens and Bambusa textilis. Among 31 batches of commercial Bambusae Caulis In Taenias, only 1 batch was authenticated as Bambusa beecheyana var. Pubescens, while the others were all adulterants, including 19 batches were identified as Phyllostachys edulis, 8 as Bambusa textilis, and 3 as other species. Furthermore, several samples were found to be mixtures of different species. CONCLUSION The method established in this study provides an accurate tool for the identification of Bambusae Caulis In Taenias original plants, laying a foundation for quality control and market regulation.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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