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OBJECTIVE To establish a quantitative analysis of multi-components by single marker (QAMS) for the simultaneous determination of hypoxanthine, vanillic acid, magnolithine, demethyleneberberine, Columbamine, Jatrorrhizine hydrochloride, coptisine hydrochloride, palmatine hydrochloride and berberine hydrochloride in Xiaoxianxiong decoction. METHODS The separation was performed on a Spursil C18 column (4.6 mm×250 mm, 5 μm) with acetonitrile and 0.05 mol·L-1 potassium dihydrogen phosphate solution plus phosphoric acid adjusted to pH=4.0 as the mobile phases. Detection wavelength: 0-20 min: 290 nm; 20-45 min: 300 nm, column temperature 30 ℃, injection volume 10 μL. Using berberine hydrochloride as the internal reference material, the relative correction factors of the remaining eight components were calculated and the content of each component was calculated, and the results were compared with those of the external standard method (ESM) and the QAMS method. The differences between the results of the external standard method (ESM) and QAMS method were compared. RESULTS The nine components of Xiaoxianxiong decoction showed a good linear relationship with peak area in a certain concentration range (r>0.998 0), with the average spiked recoveries of 94.36%-105.57% and the RSD was 0.52%-3.48%, and the results of component contents determined by QAMS and ESM were not statistically significant. CONCLUSION The results of the established QAMS for Xiaoxianxiong decoction are accurate and reliable, and the method is simple and easy to be applied to the quality control of Xiaoxianxiong Decoction.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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