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OBJECTIVE To establish a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for simultaneous determination of tobramycin and dexamethasone in artificial tears, and detect drug use concentration in human tears and in vitro release. METHODS Zorbax Eclipse plus-C8 column was used with 2 mmol·L-1 ammonium acetate aqueous solution as mobile phase A and 90% methanol aqueous solution as mobile phase B for gradient elution. The flow rate was 0.5 mL·min-1, the column temperature was maintained at 40 ℃, and the injection volume was 2 μL. A mass spectrometer was used with ESI ionization mode, MRM mode, and positive ion mode. RESULTS Tobramycin and dexamethasone showed good linearity in the concentration range of 200-5 000 ng·mL-1, and the lower limit of quantification was 200 ng·mL-1. CONCLUSION The established method is rapid, efficient, accurate and sensitive, and can be used for the detection of drug concentration in tears and in vitro release test of tobramycin and dexamethasone eye drops.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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