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OBJECTIVE To explore the characteristics of the inhibitory effect of low concentrations of deguelin on the proliferation of activated T cells. METHODS Human peripheral blood mononuclear cells were isolated by Ficoll-Hypaque density gradient centrifugation, human T cells were purified using immunomagnetic beads, and T cells were activated with Anti-CD3/CD28 antibodies. Flow cytometry was used to detect T cell survival rate, proliferation index, apoptosis progression, CD25 expression level, and cell division ratio; ELISA was used to detect cytokines IL-2, IL-4, IL-6, IL-17, and IFN-γ secretion levels. RESULTS Low concentrations of deguelin inhibit T cell proliferation activated by anti-human CD3/CD28 antibodies, with an IC50 of (73±12) nmol·L-1, and a concentration of 400 nmol·L-1 has no cytotoxicity. Low concentrations of deguelin do not affect the expression of CD25 and secretion of IL-2 in activated T cells but increases the proportion of G0/G1 phase cells. Low concentrations of deguelin promote the secretion of anti-inflammatory cytokine IL-4 and inhibit the production of pro-inflammatory cytokines IL-6, IL-17, and IFN-γ in T cells. CONCLUSION Low concentrations of deguelin significantly inhibit T cell proliferation in the G0/G1 phase and effectively suppress the secretion of pro-inflammatory cytokines, suggesting its potential role in the treatment of autoimmune diseases.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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