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Analysis of Chemical Constituents of Gukang Capsules by UPLC-QE-Orbitrap-MS/MS
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Yingjie XU1, 2, Hongyun HUANG1, 2, Yang WANG3, Chunhua LIU1, Lin ZHENG3, Jia SUN3, *, Yongjun LI1, 2, 3, *
Chinese Pharmaceutical Journal | 2025, 60(3) : 290 - 301
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Chinese Pharmaceutical Journal | 2025, 60(3): 290-301
Analysis of Chemical Constituents of Gukang Capsules by UPLC-QE-Orbitrap-MS/MS
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Yingjie XU1, 2, Hongyun HUANG1, 2, Yang WANG3, Chunhua LIU1, Lin ZHENG3, Jia SUN3, *, Yongjun LI1, 2, 3, *
Affiliations
  • 1 State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University Engineering Research Center for the Development and Application of Ethnic Medicine and TCM(Ministry of Education), Guiyang 550004, China
  • 2 College of Pharmacy, Guizhou Medical University,Guiyang 550004,China
  • 3 Guizhou Provincial Key Laboratory of Pharmaceutics, National Engineering Research Center of Miao's Medicines, Guizhou Medical University, Guiyang 550004, China
Published: 2025-02-08 doi: 10.11669/cpj.2025.03.012
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OBJECTIVE To study the chemical composition of Gukang Capsules by ultra-high performance liquid chromatography-quadrupole/electrostatic field orbitwell high resolution mass spectrometry(UPLC-QE-Orbitrap-MS/MS).METHODS The analysis of 70%methanol extract of the drug was performed on a 45 ℃ thermostatic Hypersil GOLD C18 column(2.1 mm×100 mm,1.9 μm)with mobile phase consisting of 0.1%formic acid solution-acetonitrile gradiently eluted at 0.3 mL·min-1,and the injection volume was 1 μL.The information of the chemical constituents of Gukang Capsules was acquired in positive and negative ion modes by electrospray ion source,and the chemical composition was analyzed and identified by software combined with control comparison,database matching,literature reports and mass spectrometry rules. RESULTS A total of 137 compounds were identified from Gukang Capsules,mainly including flavonoids,saponins,organic acids and coumarins,etc.,among which 27 compounds were identified by comparison with reference substances and their origins of medicinal materials were determined.CONCLUSION In this study,the main chemical composition of Gukang Capsules was identified by this rapid and efficient analytic method,which laid a foundation for further research on pharmacodynamic substance base and quality control.

Gukang Capsule  /  UPLC-QE-Orbitrap-MS/MS  /  flavonoid  /  saponin
Yingjie XU, Hongyun HUANG, Yang WANG, Chunhua LIU, Lin ZHENG, Jia SUN, Yongjun LI. Analysis of Chemical Constituents of Gukang Capsules by UPLC-QE-Orbitrap-MS/MS[J]. Chinese Pharmaceutical Journal, 2025 , 60 (3) : 290 -301 . DOI: 10.11669/cpj.2025.03.012
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Year 2025 volume 60 Issue 3
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doi: 10.11669/cpj.2025.03.012
  • Receive Date：2024-08-13
  • Online Date：2025-10-29
  • Published：2025-02-08
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  • Received：2024-08-13
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Affiliations
    1 State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University Engineering Research Center for the Development and Application of Ethnic Medicine and TCM(Ministry of Education), Guiyang 550004, China
    2 College of Pharmacy, Guizhou Medical University,Guiyang 550004,China
    3 Guizhou Provincial Key Laboratory of Pharmaceutics, National Engineering Research Center of Miao's Medicines, Guizhou Medical University, Guiyang 550004, China
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小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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