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OBJECTIVE To explore the supercritical CO2 extraction process of Curcuma longa L. and its effects on alleviating alcohol intoxication and protecting the liver. METHODS The extraction yield, curcumin, and α-turmerone contents were used as evaluation indicator. Single-factor experiments and orthogonal experimental designs were employed to optimize process parameters such as the amount of co-solvent, extraction pressure, extraction temperature, extraction time, separation vessel pressure, and temperature. The alcohol alleviating effects, pathways, and liver protection of Curcuma longa L. Supercritical CO2 extract were evaluated using mouse alcohol intoxication models, rat alcohol intoxication models, and mouse subacute alcohol-induced liver injury models. The results indicated that the optimal process for Curcuma longa L. RESULTS Supercritical CO2 extraction included: an extraction pressure of 30 MPa, extraction temperature of 55 ℃, using ethanol as a co-solvent at a weight equal to that of the medicinal material, an extraction time of 2 hours, maintaining consistent pressure in the separation vessel and storage tank, a separation vessel temperature of 40 ℃, and ethanol recovery through depressurization at 50 ℃. Furthermore, the supercritical extract significantly reduced the sleep time of mice after alcohol intoxication and improved their wake-up experiment (P<0.01), increased the ADH level in rats (P<0.01), and reduced the levels of TC, LDL-C, and TBIL in the serum of mice in the subacute liver injury model (P<0.01). CONCLUSION The preparation process developed in this study has a high extraction rate, is stable and feasible, and the supercritical CO2 extract produced exhibited good alcohol alleviating effects and reduces subacute alcohol-induced liver injury.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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