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Identification and Analysis of Bacillus Species Contaminating Traditional Chinese Medicine Based on gyrB Gene
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Yongqi GAN1, Cheng LIU2, Bin ZHU1, Lanyan FAN1, Jun NONG1, Jie HUANG1, Wenchao LING1, Manman LU3, *
Chinese Pharmaceutical Journal | 2025, 60(3) : 244 - 249
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Chinese Pharmaceutical Journal | 2025, 60(3): 244-249
Identification and Analysis of Bacillus Species Contaminating Traditional Chinese Medicine Based on gyrB Gene
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Yongqi GAN1, Cheng LIU2, Bin ZHU1, Lanyan FAN1, Jun NONG1, Jie HUANG1, Wenchao LING1, Manman LU3, *
Affiliations
  • 1 Guangxi Institute for Drug Control,Nanning 530021, China
  • 2 School of Pharmaceutical, Guangxi Medical University, Nanning 530021, China
  • 3 Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530004, China
Published: 2025-02-08 doi: 10.11669/cpj.2025.03.006
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OBJECTIVE To indentify and analyze Bacillus species and their characteristics contaminating traditional Chinese medicine. METHODS Screening for a reaction system more suitable for gyrB gene amplification conditions and sequencing Bacillus species in contaminated traditional Chinese medicine preparations, medicinal herbal, herbal pieces and extracts. Analyzing the types and homogeneity of contaminating bacteria through comparing gene sequences and constructing a phylogenetic tree. RESULTS The results indicate that the prevalence of Bacillus subtilis as the most common contaminating bacterium across various sample types, followed by Bacillus velezensis, Bacillus megaterium, Bacillus licheniformis and Bacillus amyloliquefaciens. Additionally, pathogenic bacterium Bacillus cereus was also detected in this analysis. The gyrB gene demonstrates good discriminatory power for various Bacillus species and subspecies, effectively distinguishing same contaminating strains relationships among diverse production enterprises, showing relevance of sources. CONCLUSION To ensure the quality and safety of traditional Chinese medicine products, production enterprises must enhance control measures at the source during the production process and select appropriate sterilization processes tailored to the characteristics of traditional Chinese medicine varieties.

Bacillus  /  gyrB gene  /  traditional Chinese medicine  /  microbial identification
Yongqi GAN, Cheng LIU, Bin ZHU, Lanyan FAN, Jun NONG, Jie HUANG, Wenchao LING, Manman LU. Identification and Analysis of Bacillus Species Contaminating Traditional Chinese Medicine Based on gyrB Gene[J]. Chinese Pharmaceutical Journal, 2025 , 60 (3) : 244 -249 . DOI: 10.11669/cpj.2025.03.006
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Year 2025 volume 60 Issue 3
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Article Info
doi: 10.11669/cpj.2025.03.006
  • Receive Date：2024-05-07
  • Online Date：2025-10-29
  • Published：2025-02-08
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  • Received：2024-05-07
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Affiliations
    1 Guangxi Institute for Drug Control,Nanning 530021, China
    2 School of Pharmaceutical, Guangxi Medical University, Nanning 530021, China
    3 Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530004, China
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https://castjournals.cast.org.cn/joweb/zgyxzz/EN/10.11669/cpj.2025.03.006
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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