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OBJECTIVE To establish a method for the quantitative detection of bacterial endotoxin in glutamic acid and verify its feasibility. METHODS Verification test of the reliability of bacterial endotoxin standard curve, interference test of calcium ion buffer and sodium ammonium hydrogen phosphate used in pre-treatment, and interference test of test product and additional endotoxin recovery test were carried out in sequence by using limulus reagents from two manufacturers according to the standard of kinetic chromogenic assay in bacterial endotoxin inspection method (General Chapter 1143 in Chinese Pharmacopoeia2020 Edition). A method for the detection of bacterial endotoxin in glutamic acid was successfully established, and three batches of samples were quantitatively detected by this method. RESULTS Glutamic acid was dissolved with sodium ammonium hydrogen phosphate together to the concentration of 20 mg·mL-1, then diluted by calcium ion buffer to the concentration of 5 mg·mL-1, which had no interference effects on bacterial endotoxin test. The contents of endotoxin in three batches of samples were all lower than the limit of 12 EU·g-1, the RSD values were all lower than 10%, and the endotoxin recovery rates were in the range of 50% to 200%, which met the requirements. CONCLUSION The bacterial endotoxin test method established in this study can be used to detect the bacterial endotoxin in glutamic acid and control its quality.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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