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OBJECTIVE To establish HPLC fingerprint and multi-component content determination method for Linderae Radix, and explore the quality of Linderae Radix from different origins by combining chemical pattern recognition method, providing scientific basis for the quality control and further development of Linderae Radix. METHODS High-performance liquid chromatography(HPLC) was employed, using acetonitrile-0.1% triethylamine aqueous solution(adjusted to pH=3.0 with acetic acid) as the mobile phase for gradient elution, to establish the fingerprint of Linderae Radix. The quality of Linderae Radix from different areas was explored through chemical pattern recognition methods, such as similarity evaluation, cluster analysis(HCA), principal component analysis(PCA), and orthogonal partial least squares discriminant analysis(OPLS-DA). RESULTS The established fingerprint method meets the methodological requirements. A total of 17 common peaks were calibrated in the fingerprint spectra of 44 batches of Linderae Radix, and the similarity was greater than 0.8. Seven components were identified, including norisoboldine, boldine, reticuline, linderalactone, linderane, linderene and lindenanolide H. The HCA analysis results showed that the 44 batches of Linderae Radix were classified into five categories. PCA analysis indicated that the cumulative variance contribution rate of the first four principal components was 80.123%. OPLS-DA analysis showed that linderane, linderene, norisoboldine, and linderalactone were identified as differential chemical components of Linderae Radix. The content determination results indicate that “Tiantai Linderae Radix” has significantly higher levels of linderane (P<0.001), linderene (P<0.001), and linderalactone (P<0.01) compared with Linderae Radix from other regions. CONCLUSION The established fingerprint and content determination method is reliable and stable, providing a reference for the quality evaluation of Linderae Radix and the multi-index quality control of related preparations.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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