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OBJECTIVE To investigate the protective effect and mechanism of salvianolic acid A (SAL-A) on mouse brain microvascular endothelial (b.End.3) cell apoptosis and oxidative stress injury after oxygen-glucose deprivation/reoxygenation (OGD/R). METHODS OGD/R injury was established with b. End.3 cells for injury model. Cell apoptosis was detected by Annexin V-fluorescein isothiocyanate (Annexin V-FITC) probe and propidium iodide (PI) staining; Reactive oxygen species (ROS) generation was detected by 2,7-dichlorofluorescein diacetate (DCFH-DA) probe; Mitochondrial membrane potential was detected by JC-1 staining. The expression of proteins related to apoptosis, oxidative stress, and Akt/GSK3β/Nrf2 pathway was detected by Western Blot. RESULTS Compared with the control group, the expression of anti-apoptotic proteins, mitochondrial membrane potential, expression of related antioxidant proteins and Akt/GSK3β/Nrf2 pathway were significantly decreased in the OGD/R group (P<0.05). In addition, the number of apoptotic cells, the expression of apoptosis-inducing proteins, and the production of intracellular ROS were significantly increased in the OGD/R group (P<0.05). Whereas, the SAL-A treatment can improve OGD/R-induced cell apoptosis and oxidative stress, the expression of Akt/GSK3β/Nrf2 pathway were significantly increased in the SAL-A-10 group. CONCLUSION SAL-A inhibited OGD/R-induced apoptosis and oxidative stress injury in b.End.3 cells, and the mechanism may be related with affecting the Akt/GSK3β/Nrf2 pathway.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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