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OBJECTIVE To study the chemical constituents of ethyl acetate extract from Moringa oleifera leaves. METHODS The chemical constituents of ethyl acetate extract from Moringa oleifera leaves were isolated and purified by chromatographic methods. The structures of isolated compounds were identified by MS and NMR data. DPPH and ABTS were used to test the antioxidant activities of the compounds in vitro. RESULTS Fourteen compounds were isolated from ethyl acetate extract of Moringa oleifera leaves and identified as icariside B1 (1), (3S)-O-β-D-glucopyranosyl-6-[3-oxo-(2S)-butenylideny]-1, 1, 5-trimethylcyclohexan-(5R)-ol (2), icariside B2 (3), 9-hydroxy-megastigma-4, 7-dien-3-one-9-O-β-D-glucopyranoside (4), tectorigenin (5), tectoridin (6), iristectorin A (7), iristectorin B (8), 5-hydroxy-2-hydroxymethylpyridine (9), androsin (10), 3, 4, 5-trimethoxyphenyl-1-O-β-D-glucopyranoside (11), 1-O-(4-hydroxymethylphenyl)-α-L-rhamnopyranoside (12), benzyl-O-β-D-glucopyranoside (13) and methyl 2-[4-(α-L-rhamnopyranosyl) phenyl] acetate (14), respectively. Among them, compounds 6, 7 and 8 had certain antioxidant activities, but the activities were weaker than that of vitamin C as positive control. CONCLUSION Compounds 2-11 are isolated from Moringa genus for the first time.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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