Full
Outline
To evaluate the purification process of Weixuening mixture extract by alcohol precipitation method and membrane separation method.
In this study, the Weixuening mixture extract was used as the research object, and physicochemical parameters, solid removal rate, and the transfer rates of indicative components were used as the indexes to evaluate the two different refining processes of Weixuening mixture extract, i.e., alcohol precipitation method and membrane separation method. By investigating the influences of different molecular weight cut-offs, operating pressure, and extracting solution concentration on membrane separation index and membrane flux, the best purification process was selected.
The physicochemical parameters of the membrane separation method were significantly better than those of the alcohol precipitation method. The transfer rates of the indicative components of the alcohol precipitation solution such as catechin, epicatechin, polydatin, resveratrol, and quercetin, respectively, were 82.34%, 83.31%, 79.06%, 77.51%, and 80.16%, respectively. The solid removal rate was 64.75%. The best process for membrane separation was as follows: the membrane pressure was 0.5 MPa; the extract concentration was 0.11 g·mL-1; the molecular weight cutoff was 30 kDa. The transfer rates of the indicative components such as catechin, epicatechin, polydatin, resveratrol, and quercetin in the membrane separated solution were 83.44%, 84.32%, 80.42%, 83.36%, and 83.58%, respectively. The membrane flux was 10.57 mL·m-2·min-1 and the solid removal rate was 65.96%.
Compared with the alcohol precipitation method, the membrane separation method is more conducive to the refining of the extract of Weixuening mixture, which lays the foundation for the industrial production of Weixuening mixture.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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