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Outline
To explored the inhibitory effect as well as the mechanism of bicyclol on pulmonary fibrosis in silicotic rats.
A silicotic rat model was established by intratracheal drip of silicon dioxide (SiO2) suspension. Hematoxylin-eosin (HE) and Masson staining of rat lung tissues were performed for the exploration of the anti-fibrotic effect of bicyclol. Immunofluorescence (IF) and immunohistochemical (IHC) staining were used to observe macrophages aggregation and activation in rat lungs. Meanwhile, Western Blot assay was performed for further verification in cultured cells in vitro.
Both macrophage aggregation and nodule formation were observed in rat lungs by HE staining 28 days after intratracheal drip, indicating successful establishment of the silicotic rat model. Compared with the control group, both pulmonary fibrosis and macrophage aggregation in lungs of silicotic rats with bicyclol treatment were suppressed significantly using HE and Masson staining. IF and IHC staining showed downregulation of the expression of both arginase-1 (Arg1) and inducible nitric oxide synthase (iNOS) in macrophages of silicotic rat lungs by bicyclol. Furthermore, inhibition of the expression of Arg1 and iNOS by bicyclol in RAW264.7 cells stimulated with SiO2 was observed using Western blot assay.
Bicyclol suppresses macrophages aggregation and activation via down regulating Arg1 and iNOS, resulting in a slow-down of the process of pulmonary fibrosis in silicotic rats, which is likely to be a novel strategy for the treatment of silicosis.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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