Full
Outline
To establish the UPLC fingerprints and multi-components content determination method of Cistanches herba formula granules and ultrafine granular power, and to provide a basis for their quality evaluation.
The separation was achieved by using a ACQUITY UPLC BEH C18 (100 mm×2.1 mm, 1.7 μm) column via gradient elution with the mobile phase consisting acetonitrile and 0.1% phosphoric acid water. The flow rate was 0.3 mL·min-1 and the detection wavelength was set at 235 nm; the column temperature was 35 ℃ and the injection volume was 1 μL.
The UPLC fingerprints of Cistanches herba formula granules and ultrafine granular power were established, in which 10 common peaks were determined, and 6 components of geniposidic acid, caffeic acid, echinacoside, acteoside, tubuloside A, and isoacteoside were identified. The contents of these 6 components were determined. Using the developed method, the components were well separated, and had wide linear ranges with good linear relationships. The stability, repeatability and recovery rate met the requirements. Using cluster analysis and principal component analysis methods, Cistanches herba formula granules and ultrafine granular power can be distinguighed and identified.
The UPLC fingerprints combined with multi-components content determination method are simple, accurate, and reproducible. It can quickly identify the formula granules and ultrafine granular power of Cistanches herba and evaluate their internal quality, providing a method reference for improving the quality control of formula granules and ultrafine granular power of Cistanches herba.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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