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Outline
LPS induces RAW264.7 cells to become an inflammation model, and uses transcriptomics and proteomics techniques to explore the anti-diabetic chronic inflammation mechanism of berberine.
After RAW264.7 cells induced by LPS (0.1 μg·mL-1) were incubated with berberine (1 μg·mL-1), protein and RNA were extracted, and proteomics and transcriptomics analysis were performed. Proteins and genes that were differentially expressed were found, and gene ontology (GO) enrichment analysis and Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis were performed. STRING was used to draw interaction diagrams (PPI) to perform real-time fluorescence quantification of common differentially expressed proteins and genes PCR for verification.
By comparing the model group with the berberine group, there are a total of 1 801 differentially expressed genes in transcriptomics, and 11 differentially expressed genes, such as Pdia4, Itgam, Hsp90b1, Prdx5, Pdia6, Myc, Fosl1, Il10, Ccl2, Lpin2 and Pik3r2, are related to inflammation. Proteomics obtained a total of 83 differentially expressed proteins, and 10 differentially expressed proteins were found, including Hsp90b1, Pdia6, Prdx5, Pdia4, Itgam, Stat1, Pgm2, Ptgs1, Nos2, Actb, are related to inflammation. There are 5 common differentially expressed proteins and genes.
Berberine may achieve anti-inflammatory effects by reducing the release of inflammatory factors, while reducing Hsp90b1, Pdia6, Prdx5, Pdia4, Itgam, PTGS1, NOS2 and IL-10, and increasing the production of Pik3r2 and LPIN 2 genes to further relieve abnormal metabolism and insulin resistance as well as reducing oxidative damage to the endoplasmic reticulum to treat diabetes.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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