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Comparative study of immune responses induced by MDCK cell-based and egg-based influenza vaccines in mice
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Zhe-gang ZHANG1, 2, Xing-hang LI2, Jia-you ZHANG1, Wei ZHAO2, Chen LIU2, Tian HAN2, Lu-yao YAN1, Zhi-wu XIA2, Chang-gui LI3, Xiao-ming YANG1, 4
Chinese Journal of New Drugs | 2021, 30(6) : 514 - 519
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Chinese Journal of New Drugs | 2021, 30(6): 514-519
Comparative study of immune responses induced by MDCK cell-based and egg-based influenza vaccines in mice
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Zhe-gang ZHANG1, 2, Xing-hang LI2, Jia-you ZHANG1, Wei ZHAO2, Chen LIU2, Tian HAN2, Lu-yao YAN1, Zhi-wu XIA2, Chang-gui LI3, Xiao-ming YANG1, 4
Affiliations
  • 1Wuhan Institute of Biological Products Co., Ltd., Wuhan 430207, China
  • 2National Institute of Engineering Technology Research in Combination Vaccines, Wuhan 430207, China
  • 3National Institute for Food and Drug Control, Beijing 100050, China
  • 4China National Biotec Group, Beijing 100029, China
Published: 2021-03-30
Outline
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Objective:

To compare the difference of immune response induced by egg-based and cell-based H1N1 influenza vaccines.

Methods:

C57BL/6 mice were injected subcutaneously with H1N1 influenza vaccines produced from eggs or MDCK cells, respectively. The cellular and humoral immune responses induced by two type of vaccines were detected by using methods such as serological test, intracellular cytokine staining and multiplex cytokine detection assay.

Results:

In terms of humoral immunity, the serum titer of the cellular vaccine group was basically the same as that of the chicken embryo vaccine group. As to cellular immunity, the secretion level of immune-related cytokines in the cellular vaccine group was more significant.

Conclusion:

Due to the different culture media, the two monovalent influenza vaccines have certain differences in the induction of immune response. Cellular vaccine has the advantage of inducing multiple immune responses, and the combination of other advantages of cellular technology indicates that the use of cellular technology will become a trend to produce influenza vaccine. This study is helpful to evaluate the immune level in mice induced by monovalent influenza vaccines prepared with different substrates.

H1N1 influenza vaccines  /  MDCK cell-based influenza vaccine  /  egg-based influenza vaccine  /  immunogenicity
张哲罡, 李兴航, 张家友, 赵巍, 刘琛, 韩天, 闫璐瑶, 夏志武, 李长贵, 杨晓明. MDCK细胞基质与鸡胚基质单价流感疫苗在小鼠中诱导的免疫反应的比较研究. 中国新药杂志, 2021 , 30 (6) : 514 -519 .
Zhe-gang ZHANG, Xing-hang LI, Jia-you ZHANG, Wei ZHAO, Chen LIU, Tian HAN, Lu-yao YAN, Zhi-wu XIA, Chang-gui LI, Xiao-ming YANG. Comparative study of immune responses induced by MDCK cell-based and egg-based influenza vaccines in mice[J]. Chinese Journal of New Drugs, 2021 , 30 (6) : 514 -519 .
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Year 2021 volume 30 Issue 6
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Article Info
  • Online Date：2026-10-29
  • Published：2021-03-30
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History
  • Accepted：2020-07-20
Affiliations
    1Wuhan Institute of Biological Products Co., Ltd., Wuhan 430207, China
    2National Institute of Engineering Technology Research in Combination Vaccines, Wuhan 430207, China
    3National Institute for Food and Drug Control, Beijing 100050, China
    4China National Biotec Group, Beijing 100029, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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