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Outline
To explore the protective effect of fermented Cordyceps Sinensis powder (Cs-4) on renal function of diabetic kidney disease rats and preliminarily investigate the mechanism.
The rat model of diabetic kidney disease was replicated by high-sugar and high-fat diet feeding combined with intraperitoneal injection of streptozotocin (40 mg·kg-1). The model rats were randomly divided into model group, low (300 mg·kg-1), medium (600 mg·kg-1) and high dose (1 200 mg·kg-1) of Cs-4 groups and positive drug group (irbesartan, 15 mg·kg-1). At the same time, the normal group (receiving ordinary diet) and the high-sugar and high-fat groups (receiving high-sugar and high-fat diet) were set up, and they were given intraperitoneal injection of the same dose of citrate buffer. Rats in each group were administered by intragastric (ig) administration once a day for 8 consecutive weeks. At the end of the experiment, urine was collected within 24 hours, and the fasting plasma glucose level was measured. Serum and kidney were collected, and kit method was used to detect blood urea nitrogen, serum creatinine, superoxide dismutase, malondialdehyde, total cholesterol, triglycerides, low density lipoprotein, high density lipoprotein, interleukin 6, interleukin 8, tumor necrosis factor α, high sensitivity c-reactive protein, free fatty acid, glycated serum protein, monocyte chemotactic protein 1, transforming growth factor β1 in serum and urinary creatinine, urinary protein, microalbuminuria content of rats in each group, and pathological and morphological changes of kidney tissue were observed by hematoxylin-eosin staining.
Compared with the model group, all dose groups of Cs-4 reduced kidney weight, kidney weight/body weight ratio, fasting plasma glucose, blood urea nitrogen, serum creatinine, urinary protein, microalbuminuria, total cholesterol, and low density lipid, malondialdehyde, interleukin 6, interleukin 8, tumor necrosis factor α, high-sensitivity c-reactive protein, free fatty acids, glycated serum protein, monocyte chemotactic protein 1, transforming growth factor β1 levels and increased body weight, urinary creatinine, creatinine clearance rate, high density lipoprotein, and superoxide dismutase levels to varying degrees (P<0.05, P<0.01), and the high-dose group of Cs-4 had a more significant effect, and could improve the morphological damage of kidney tissue to varying degrees.
Cs-4 has a certain protective effect on kidney damage in diabetic kidney disease rats, and its mechanism may be related to reducing blood glucose, regulating blood lipids, improving oxidative stress and inflammation levels, and inhibiting the degree of renal fibrosis.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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