Full
Outline
To investigate the effects of five missing components in the Decoction of Wuling Powder on astrocyte edema and aquaporin4 (AQP4) expression after oxygen glucose deprivation and reoxygenation.
Astrocytes were divided into normal group, model group, cinnamaldehyde (CIN) group, alisol B 23-acetate (ALB) group, ergosterol (ERG) group, pachymic acid (PA) group, atractylenolide Ⅰ (ATR) group, medicinal chemical composition (MCC) group and ozagrel (ozagrel, OZA) group. MTT method was used to evaluate the drug's inhibitory rate on the cells, the lactate dehydrogenase (LDH) leakage rate was used to reflect the degree of cell damage, and Western blot method was used to determine the expression change of AQP4.
After 2 hours of oxygen glucose deprivation and 6 hours of reoxygenation, the LDH leakage rate was significantly increased (P<0.01). ALB at a concentration of 10 μg·mL-1, PA and ATR at 0.01 μg·mL-1, and MCC at 0.1 μg·mL-1 could significantly reduce the increase in the leakage rate of reoxygenation LDH after oxygen glucose deprivation (P<0.01). The expression of AQP4 under oxygen and glucose deprivation and reoxygenation conditions showed significant differences among the groups. Compared with the normal group, the expression of AQP4 in the model group was significantly increased after oxygen glucose deprivation under reoxygenation conditions (P<0.01). Compared with the model group, the expression trends of AQP4 in the CIN, ALB, ERG, PA, ATR and MCC groups under reoxygenation conditions after oxygen glucose deprivation were similar, but the AQP4 protein expressions in the CIN, ALB, ERG, PA, ATR, and MCC groups were all significantly reduced (P<0.01).
The five main missing components in the decoction of Wuling Powder can significantly reduce the astrocyte edema after 2 hours of oxygen deprivation and 6 hours of reoxygenation, which may be related to the decrease of AQP4 protein expression level.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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