Full
Outline
To assess the protective effect and mechanism of mosloflavone against heart damage induced by hypobaric hypoxia (HH) in mice.
60 male BALB/c mice were randomly divided into four groups: control group, hypoxia group, rutin group and mosloflavone group. The mice were orally treated for 5 days with drugs or water and then exposed to HH equivalent to 8 000 m for 12 h. Serum concentrations of lactic dehydrogenase (LDH), creatine kinase (CK), aspartate transaminase (AST) and cardiac troponin I (cTnI) were assessed. The levels of reactive oxygen species (ROS), malondialdehyde (MDA) and glutathione (GSH), as well as the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and ATPase, in heart were estimated. The expression of Bax and Bcl-2 were evaluated by Western blot analysis.
Compared with control group, the levels of LDH, CK, AST and cTnI in serum in hypoxia model group significantly increased. In addition, HH markedly increased the contents of ROS and MDA and decreased the GSH contents and the activities of SOD, CAT, GSH-Px and ATPase in heart. Furthermore, HH significantly unregulated the expression of Bax and downregulated the expression of Bc1-2. In contrast, mosloflavone alleviated heart damage induced by HH as evidence of reduced LDH, CK, AST and cTnI levels in serum. Prior administration of mosloflavone also decreased the ROS and MDA contents, increased GSH level, SOD, CAT, GSH-Px, Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities, downregulated the expression of Bax, as well as increased the expression ratio of Bax/Bc1-2 in heart tissue of mice.
Mosloflavone exhibited protective effect against HH induced heart injury in mice via alleviating of oxidative stress, ameliorating of energy metabolism, as well as regulating of apoptosis related proteins.
27
9
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
