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Outline
To investigate the immunoregulatory effect of Lycium barbarum polysaccharides (LBP) of different molecular weights (Mw) on RAW264.7 macrophages.
LBP with different Mw was obtained by water extraction, alcohol precipitation and chromatographic column separation. CCK8 method was used to detect the effect of LBP on proliferation of RAW 264.7 cells. The effect of LBP on cell phagocytosis was determined by neutral red method. Real-time quantitative PCR was applied to detect the mRNA expression of TNF-α, IL-6, IL-1β and iNOS. Western blot was used to detect the phosphorylation level of proteins in MAPK signaling pathway.
Two high purity homogeneous components, LBPl (Mw=1 207 kDa) and LBP2 (Mw=125 kDa) were obtained by separation and purification. Compared with the control group, LBP1 and LBP2 at different concentrations significantly promoted the proliferation and phagocytosis of macrophages (P<0.05). LBP1 and LBP2 in the concentration range of 50~200 μg·mL-1 could promote the expression of TNF-α, IL-1β, IL-6 and iNOS mRNA in macrophages in a dose-dependent manner. Western blot analysis showed that LBP1 and LBP2 induced immune response in RAW264.7 cells, which was related to the specific activation of JNK MAPK pathway. In addition, the regulatory effect of LBP1 was better than that of LBP2 on RAW264.7 cells.
The immunoregulatory effect of LBP on RAW264.7 macrophages may be related to its specific activation of JNK MAPK signal transduction pathway, and its activity is closely related to the Mw of LBP.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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