Full
Outline
To identify the related substances in mesaconine.
An HPLC-ELSD method was established using Gemini C18 column (150 mm×4.6 mm, 5 μm) with mobile phase composed of methanol-0.1% trifluoroacetic acid to separate the impurities. Forced degradation tests were designed and performed on mesaconine to specify the impurities. Ultra-high performance liquid chromatography tandem time-of-flight mass spectrometry (UPLC-TOF/MS) and triple quadrupole mass spectrometry (UPLC-MS/MS) were used for determination of exact mass and product ions of impurities. The structures of impurities and fragmentation regularity were then figured out.
A total of four impurities were detected and identified in mesaconine, including three impurities derived from synthesis process and a degradation product.
UPLC-MS/MS technology can effectively separate and identify the impurities in mesaconine, and the results provide a valuable reference for the manufacturing process and quality control of mesaconine.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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