Full
Outline
To investigate the protective effect of gardenoside on dementia-like mice induced by tail intravenous injection of streptozotocin (STZ).
Mice receiving STZ tail intravenous injection were randomly divided into model group (Model), low-dose gardenoside group (GL), high-dose gardenoside group (GH), and solvent control group (Vehicle). Blood glucose level was monitored every two weeks. After administration of the drug, a glucose tolerance test was conducted, and serum insulin levels were detected by ELASA. Morris water maze test was used to test the learning and memory ability of mice in each group, and Contextual fear system was used to test the ability of fear memory regression of mice in each group. Western blot was used to detect the expression of GSK-3β and phosphorylated GSK-3β (P-GSK3β) protein in hippocampus and cortical tissues.
Compared with the Vehicle group, the blood glucose level in the Model group was significantly increased (P<0.001). The spatial learning and memory function as shown by Morris water maze detection were significantly impaired (P<0.05). In the episodic fear memory experiment, there were disorder in the extinction of fear memory in mice (P<0.01) and significant decrease in serum insulin (P<0.05), while serum insulin resistance index increased significantly (P<0.001). The expression level of GSK3β remained unchanged in hippocampus and cortex, while the expression level of P-GSK3β decreased significantly (P<0.05). All abnormal indexes in the GH group were improve (P<0.05). In the GL group, some abnormal indexes were improved to a certain extent.
Gardenoside can improve spatial learning and memory impairment and fear memory extinction disorders in mice receiving STZ tail intravenous injection. The mechanism may be increasing P-GSK3β to inhibit GSK-3β activation, thereby protecting nerve cells.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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