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Optimization of the extraction process of Scutellaria baicalensis by ionic liquids with Box-behnken response surface methodology and anti-inflammatory evaluation
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Cui HAO1, 2, Li-hai ZHAI3, 4, Hong-jing DONG1, 2, Fu-min XUE1, 2, Yan CHENG1, 2
Chinese Journal of New Drugs | 2021, 30(11) : 1031 - 1037
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Chinese Journal of New Drugs | 2021, 30(11): 1031-1037
Optimization of the extraction process of Scutellaria baicalensis by ionic liquids with Box-behnken response surface methodology and anti-inflammatory evaluation
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Cui HAO1, 2, Li-hai ZHAI3, 4, Hong-jing DONG1, 2, Fu-min XUE1, 2, Yan CHENG1, 2
Affiliations
  • 1School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China
  • 2Qilu University of Technology (Shandong Academy of Sciences), Shandong Analysis and Test Center, Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Jinan 250014, China
  • 3Lunan Pharmaceutical Group Co., Ltd., Linyi 276006, China
  • 4National Chiral Pharmaceutical Engineering Technology Research Center, Linyi 276006, China
Published: 2021-06-15
Outline
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Objective:

To optimize the extraction conditions of Scutellaria baicalensis by ultrasound-assisted ionic liquid and evaluate the anti-inflammatory activity.

Methods:

The extraction method of scutellarin, baicalin, baicalein, wogonoside, wogonin and oroxylin A by high performance liquid chromatography was established. The extraction process was optimized by Box-Behnken response surface method, with the index of comprehensive score such as weight coefficient and the anti-inflammatory activity was evaluated.

Results:

The optimal extraction conditions were as follows: the concentration of ionic liquid was 20%, the ratio of material to liquid was 1∶60 mL·g-1, the extraction temperature was 35 ℃, the extraction time was 15 min. The contents of six chemicals were obtained as 0.73%, 10.05%, 2.61%, 1.30%, 0.45%, and 0.22% by the validation experiment. MTT assay showed that the cell viability significantly decreased as the concentration exceeded 50 μg·mL-1. Compared with LPS group, the extraction at different concentrations significantly inhibited the release of NO (P<0.01), and the IC50 value was 17.07 μg·mL-1.

Conclusion:

The extraction process is simple, feasible, green, high efficiency and highly reproducible and the total flavonoids of Scutellaria baicalensis have obvious anti-inflammatory activity.

Scutellaria baicalensis  /  Box-Behnken response surface methodology  /  ionic liquid  /  anti-inflammatory evaluation
郝翠, 翟立海, 董红敬, 薛富民, 程燕. Box-Behnken响应面法优化超声波辅助离子液体提取黄芩化学成分方法及抗炎活性评价. 中国新药杂志, 2021 , 30 (11) : 1031 -1037 .
Cui HAO, Li-hai ZHAI, Hong-jing DONG, Fu-min XUE, Yan CHENG. Optimization of the extraction process of Scutellaria baicalensis by ionic liquids with Box-behnken response surface methodology and anti-inflammatory evaluation[J]. Chinese Journal of New Drugs, 2021 , 30 (11) : 1031 -1037 .
Appendix
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Year 2021 volume 30 Issue 11
54
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Article Info
  • Online Date：2026-10-28
  • Published：2021-06-15
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History
  • Accepted：2020-09-26
Funding
Affiliations
    1School of Pharmaceutical Sciences, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, China
    2Qilu University of Technology (Shandong Academy of Sciences), Shandong Analysis and Test Center, Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Jinan 250014, China
    3Lunan Pharmaceutical Group Co., Ltd., Linyi 276006, China
    4National Chiral Pharmaceutical Engineering Technology Research Center, Linyi 276006, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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