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Outline
To examine the effect of Shuganning Zhusheye on the growth of human hepatic stellate cells LX2 (HSC-LX2), and to explore its anti-fibrosis mechanism.
The HSC-LX2 cells were treated with Shuganning Zhusheye and the absorbance of the cells was measured by the CCK8 assay to calculate the cell proliferation rate. The expression levels of mRNA and protein such as collagen Ⅰ, collagen Ⅲ and TGF-β were detected by fluorescence quantitative PCR and Western Blot. The effect of Shuganning Zhusheye on the cell cycle distribution and apoptotic rate of HSC-LX2 cell was detected by flow cytometry.
Compared to the untreated group, the inhibition rates of the low, medium and high concentrations of Shuganning Zhusheye on HSC-LX2 cells for 120 h were 14.27%, 16.37% and 18.67% respectively (P<0.05). The quantitative results showed that the mRNA levels and the protein expression levels of collagen Ⅰ and collagen Ⅲ in HSC-LX2 cells were lower than those in the untreated group (P<0.05). Shuganning Zhusheye blocked the cycle of HSC-LX2 cells in G2/M phase and induced the early and late apoptosis of the cells with a maximum apoptosis rate of 2.69%. And the effect of inducing apoptosis was concentration-dependent (P<0.05).
Shuganning Zhusheye inhibited cell proliferation by blocking the cell cycle, and promoted the early and late cell apoptosis. At the same time, it downregulated the expression levels of mRNA and protein regarding collagen Ⅰ and collagen Ⅲ, so as to play an anti-fibrosis effect.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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