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Outline
To observe the expression of endoplasmic reticulum related protein in hippocampus of VD model rats, determine whether endoplasmic reticulum stress (ERS) plays an important role in the progress of VD, and observe whether different doses of butylphthalide (NBP) has protective effect by regulating the expressions of markers related to ERS.
Grouping and model preparation were carried out in accordance. A Morris water maze experiment was conducted after giving NBP intervention. Western Blot and real time-PCR assay were used for evaluating the expression of endoplasmic reticulum related protein after bilateral common carotid artery occlusion among sham group, model group and different doses of NBP-treated groups.
The escape latency of model group was significantly longer than the sham group and NBP-treated groups on day 2 (P<0.05). Compared with the sham group, the escape latency of the model group was significantly longer on days 3~5 (P<0.05). We detected the protein expression of endoplasmic reticulum related protein. Except for GRP78 in 30 mg·kg-1 NBP group, the expressions of other proteins in rats treated with NBP significantly decreased compared with the model group (P<0.05). NBP of 120 mg·kg-1 significantly down-regulated the expression of all the proteins except for XPB1 and elF2α (P<0.05). Except for proteins, we also detected the mRNA expression of endoplasmic reticulum related protein. The mRNA levels of CHOP and elF2α were significantly increased in NBP30 group compared with the model group (P<0.01) and there was no significant difference between NBP60 group and the model group. Besides CHOP and elF2α, all the other markers were significantly decreased in NBP120 group compared with the model group (P<0.01). The NBP120 group exhibited the most significant differences among the treated groups except for caspase-12 (equal in NBP120 and NBP60 groups) (P<0.01).
The markers of ERS in hippocampal tissue were activated in the VD model rats. After giving NBP, the expressions of endoplasmic reticulum related protein were down-regulated. NBP played neuroprotective effects by inhibiting apoptosis of neurons and promoting cell survival by down-regulating the markers related with ERS.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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