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Outline
To optimize the formula of long-circulation liposomes loaded with pulchinenoside B4 (PB4-L) by central composite design-response surface method and characterize the liposomes.
PB4-L were prepared by ethanol injection and the encapsulation efficiency was determined by ultrafiltration centrifugation. The formula of PB4-L was optimized by the four-factor, three-level central composite design-response surface method. The particle size, zeta potential and morphology of PB4-L were characterized by laser particle zeta-sizer and transmission electron microscope. The stability of the liposomes was evaluated.
The optimal formula of PB4-L was as follows: the phospholipid content was 2.4%, the weight ratio of phospholipids to cholesterol was 3∶1, the weight ratio of lipids to drug was 2∶1, and the DSPE-PEG2000 content was 2.4%. The encapsulation efficiency of PB4-L was 76.9%, with a mean particle size of (408.7±1.1) nm, polymer dispersity index (PDI) of 0.16, and zeta potential of (-45.2±0.2) mV. The morphology study of PB4-L showed that the liposomes were round-shaped with good dispersity. PB4-L was stable in 10% FBS-containing medium.
The formula of PB4-L was optimized by central composite design-response surface method. The liposomes were characterized by a high encapsulation efficiency, a uniform particle size distribution and a good serum stability in vitro.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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