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Simultaneous determination of five constituents in Artemisia rupestris L. by HPLC-DAD
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Muhetaer MUHEBAITI, Feng ZHAO, Maitinuer MAIWULANJIANG
Chinese Journal of New Drugs | 2021, 30(17) : 1621 - 1626
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Chinese Journal of New Drugs | 2021, 30(17): 1621-1626
Simultaneous determination of five constituents in Artemisia rupestris L. by HPLC-DAD
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Muhetaer MUHEBAITI, Feng ZHAO, Maitinuer MAIWULANJIANG
Affiliations
  • State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China
Published: 2021-09-15
Outline
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Objective:

To develop an HPLC-DAD method for the simultaneous determination of the contents of 5 components (isochlorogenic acid A, isochlorogenic acid C, linarin, rupestonic acid, and chrysosptertin B) in Artemisia rupestris L.

Methods:

The chromatographic separation was achieved on a InerSustainR C18 (250 mm×4.6 mm, 5 μm) column with acetonitrile (A)-0.2% phosphoric acid (B) as mobile phase at a flow rate of 1.0 mL·min-1 for gradient elution. Isochlorogenic acid A, isochlorogenic acid C, linarin, rupestonic acid and chrysosptertin B were detected at 320, 320, 334, 245, 350 nm, respectively. Injection volume was set at 10 μL. To establish an HPLC chromatographic fingerprint of Artemisia rupestris L., the similarity evaluation was carried out. The cluster analysis was performed by using SPSS 23.0 software.

Results:

There were 25 common peaks in HPLC fingerprint of 15 batches of Artemisia rupestris L. The similarities of 10 batches of samples were more than 0.85. The 10 batches of samples can be divided into 2 categories by cluster analysis.

Conclusion:

The method is simple, quick, accurate, and helpful for the quality control of Artemisia rupestris L.

Artemisia rupestris L.  /  HPLC-DAD  /  fingerprint  /  clustering analysis
穆合拜提·木合塔尔, 赵丰, 买吾兰江·买提努尔. HPLC-DAD同时检测一枝蒿药材中的5种化学成分研究. 中国新药杂志, 2021 , 30 (17) : 1621 -1626 .
Muhetaer MUHEBAITI, Feng ZHAO, Maitinuer MAIWULANJIANG. Simultaneous determination of five constituents in Artemisia rupestris L. by HPLC-DAD[J]. Chinese Journal of New Drugs, 2021 , 30 (17) : 1621 -1626 .
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Year 2021 volume 30 Issue 17
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Article Info
  • Online Date：2026-10-28
  • Published：2021-09-15
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History
  • Accepted：2021-03-18
Funding
Affiliations
    State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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