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Outline
To observe the effect of panax notoginseng saponins (PNS) on the differentiation of CD4+ T cells into regulatory T (Treg) cells and preliminarily explore its relationship with regulating cellular fatty acid oxidation metabolism.
The spleen cells of C57BL/6 mice were isolated and CD4+ T cells were cultured in vitro by magnetic beads sorting. The optimal concentration of PNS was screened by the CCK-8 method and its effect on cell viability was tested. Then the cells were divided into control group and PNS intervention groups (5, 10, 20 μg·mL-1). After corresponding drug intervention, the proportion of Treg cells was detected by using flow cytometry, the level of β-hydroxybutyric acid (BHBA) in the supernatant was measured by using ELISA, cellular carnitine palmitoyl transferase 1A (CPT-1A) mRNA expression was detected by using q-PCR, and the expression of CPT-1A protein was detected by using Western Blot method.
The differentiation ratio of Treg cells and BHBA level in the supernatants significantly increased in the PNS intervention groups (5, 10, 20 μg·mL-1). The increasing expressions of CPT-1A mRNA and protein in the cells were also observed in the PNS intervention groups.
Certain concentrations of PNS can significantly promote the differentiation of mouse CD4+ T cells into Treg cells, and its mechanism may be related with promoting the fatty acid oxidation of CD4+ T cells.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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