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Outline
A comparative study of two in vitro experimental methods detecting cytokine release was conducted for evaluating methodological choices on whether new drugs cause cytokine release.
Peripheral blood mononuclear cells were incubated with two different methods for 24 hours, after which the expression levels of four cytokines: IL-1β, IL-6, IL-8 and TNF-α were detected by cytometric bead assay.
In solid phase method, IL-1β was significantly released only in OKT3 at 5 μg·well-1 (P<0.05). Significant release of IL-6 was found in two antibodies (P<0.05). There was a significant release of IL-8 and TNF-α at all concentrations of OKT3 and 5 μg·well-1 of TGN1412 (P<0.05). While in liquid phase method, significant release of IL-6 happened in 1 and 5 μg·well-1 of OKT3, as well as 5 μg·well-1 of TGN1412 (P<0.05). There was a significant release of IL-8 at 5 μg·well-1 of TGN1412 (P<0.05). Significant release of TNF-α was found in all concentrations of OKT3 and 5 μg·well-1 of TGN1412 (P<0.05).
The solid phase method is more suitable for T cell receptor monoclonal antibody drugs, the results of which are more reliable in preclinical evaluation. The liquid phase method is simpler to operate, and its simulated environment is closer to human body, but it has higher sensitivity for monoclonal antibodies whose target is not on T cells.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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