Full
Outline
To establish the HPLC fingerprinting and quantitative method of eight characteristic components in water-decocted extract of Angelicae Dahuricae Radix, and investigate the similarities of 14 batches of Angelicae Dahuricae Radix samples.
SinoChrom ODS-BP column (250 mm×4.6 mm, 5 μm) was used for the fingerprinting and quantitative assay. The mobile phase was 0.025% phosphoric acid-acetonitrile under gradient elution mode. The column temperature was 35 ℃, the detection wavelength was 220 nm, the flow rates were 1.0 mL·min-1 for fingerprinting and 1.2 mL·min-1 for quantitative assay, respectively.
In the HPLC fingerprinting, there were 29 common peaks in 14 batches of Angelicae Dahuricae Radix, and eight characteristic components were identified (xanthotol, 5-hydroxy-8-methoxypsoralen, oxypeucedanin hydrate, byakangelicin, xanthotoxin, bergapten, isoimpinellin, imperatorin, and phellopterin). The fingerprints of the 14 batches of Angelicae Dahuricae Radix were similar and the similarities were greater than 0.980. The linear ranges of xanthotoxol, 5-hydroxy-8-methoxypsoralen, oxypeucedanin hydrate, byakangelicin, xanthotoxin, bergapten, imperatorin, and phellopterin were 3.24~162.00, 2.86~143.00, 3.10~155.00, 2.98~149.00, 3.12~156.00, 3.02~151.0, 3.16~158.00, and 2.30~115.00 μg·mg-1, respectively. The content ranges of xanthotoxol, 5-hydroxy-8-methoxypsoralen, oxypeucedanin hydrate, byakangelicin, xanthotoxin, bergapten, imperatorin, and phellopterin were 0.14~0.37, 0.26~0.39, 2.31~3.48, 1.09~2.22, 0.04~0.26, 0.19~0.34, 0.03~0.14, and 0.11~0.23 μg·mg-1, respectively for the 14 batches of Angelicae Dahuricae Radix.
The established methods of HPLC fingerprinting and quantitative analysis are reproducible, reliable, and stabile. In the future, these methods can be used for the quality control of original, processed materials and products of Angelicae Dahuricae Radix.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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