Full
Outline
To systematically investigate the dynamic tissue distribution and tumor-targeting characteristics of memory T cells in non-tumor-bearing and tumor-bearing NCG mouse models using validated qPCR and in vivo imaging techniques.
A total of 144 NCG mice were randomly divided into non-tumor-bearing control, tumor-bearing control, non-tumor-bearing treatment, and tumor-bearing treatment groups. The treatment groups received a single intravenous injection of 1×107 memory T cells per mouse, while the control groups received vehicle only. In vivo imaging was performed on days 1, 3, 7, 14, 21, and 28 post-administration. Tissues and body fluids were collected at the same time points, and the genomic distribution of memory T cells was quantified by qPCR.
In non-tumor-bearing mice, memory T cells were initially (day 1~3) distributed mainly in highly perfused organs such as the lung, spleen, and liver, followed by a rapid decline and near clearance by day 14. In tumor-bearing mice, the early distribution pattern resembled that in non-tumor-bearing animals; however, starting from day 14 onward, significant tumor enrichment was observed and persisted until day 28, accompanied by a concurrent decrease in cell concentrations in non-target tissues.
Memory T cells demonstrate a trend of rapid clearance in the absence of antigenic stimulation, whereas in the presence of tumor, they exhibit antigen-driven tumor homing and long-term persistence capabilities.
52
7
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62662699(Operation and Technical Support)
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
