Full
Outline
To establish the identification and content determination methods of Qi Feng Er Teng Granules.
TLC was used for identification and HPLC was used for content determination.
The TLC method allowed effective identification of all crude drugs in the formulation. The calibration curves of paeoniflorin, prim-O-glucosylcimifugin, calycosin-7-glucoside, 5-O-methylvisammioside, icariin, sinomenine, and astragaloside IV showed good linearity in the range of 9.01~45.1, 1.69~8.44, 1.04~5.20, 1.32~6.59, 4.35~21.7, 13.5~67.5, 101~506 μg·mL-1, respectively. The average recovery rate of sample addition was within the range of 95.0% to 105.0% (RSD<3.2%).
The TLC and HPLC methods established in this study for Qi Feng Er Teng Granules have strong specificity, high sensitivity and good reproducibility, and can be used for the quality control of Qi Feng Er Teng Granules.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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