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Objective: To investigate the mechanism of inhibitory effect of hydroxy-γ-isosanshool on estrogen synthesis in human ovarian granulosa cells.Methods:The effects of four kinds of sanshool on the proliferation of human ovarian granulosa cell line (KGN cell line) were detected by CCK-8. The effects of four kinds of sanshool on estradiol synthesis in KGN cells were detected by Elisa. Aromatase gene in KGN cells was detected by RT-qPCR, and aromatase protein and related pathway proteins were detected by Western Blot.Results:Hydroxy-γ-isosanshool had the strongest inhibitory effect on estradiol biosynthesis [IC50=(16.63±0.39) μmol·L-1], while had no significant inhibitory effect on the proliferation of KGN cells at the same concentration. The expression levels of aromatase gene and protein decreased significantly after the intervention of hydroxy-γ-isosanshool (P<0.05), and the activity of aromatase was not significantly affected. Hydroxy-γ-isosanshool significantly reduced the protein expressions of P-CERB, P-AKT and P-p38 (P<0.05).Conclusion:Hydroxy-γ-isosanshool may inhibit aromatase transcription and estrogen biosynthesis in KGN cells by inhibiting the activation of p38/MAPK and PI3K/AKT pathways and reducing the level of CREB phosphorylation, inhibit aromatase transcription, and ultimately inhibit the estrogen biosynthesis of KGN cells.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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