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Objective: To compare the effects of different extraction methods on polysaccharides from Polygoni Multiflori Radix, and to explore the differences of polysaccharides from Polygoni Multiflori Radix and Polygoni Multiflori Radix Praeparata using hot water extraction. Methods: Polysaccharides from Polygoni Multiflori Radix were extracted by hot water extraction, ultrasonic extraction and microwave extraction, respectively. The yield, total sugar content, protein content, weight-average molecular weight (Mw), monosaccharide composition and DPPH free radical scavenging ability of polysaccharides were studied and compared. Results:The results showed that the yield of polysaccharides from Polygoni Multiflori Radix by different extraction methods was as follows: microwave extraction (M-PMP) > hot water extraction (H-PMP) > ultrasonic extraction (U-PMP). The protein content of U-PMP was the highest, and the Mw was obviously different from other polysaccharides. The results of monosaccharide composition showed that the monosaccharide composition of polysaccharide from Polygoni Multiflori Radix by different extraction methods was the same, which is mainly composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose, with different molar ratio of monosaccharides. Conclusion:There are some differences in the polysaccharide components extracted by three methods, and the yield obtained from different extraction methods varied significantly, with the highest yield of M-PMP. Some differences were found in polysaccharides from raw and prepared Polygoni Multiflori Radix and Polygoni Multiflori Radix Praeparata.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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