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Objective: To optimize the parameters in separation and purification processes of Yixintai total saponins, by using failure mode and effects analysis (FMEA) combined with Box-Behnken response surface design. Methods: The total saponins of Yixintai Compound were purified with macroporous resin (MAR), and the key process parameters of the purification process were screened by FMEA. Taking the recovery rate and mass fraction of saponins as evaluation indicators, the Box-Behnken design was used to optimize the MAR purification process of Yixintai total saponins. The optimal parameters were finally obtained in separation and purification processes of Yixintai total saponins. Results: FMEA determined four influencing factors, including the mass concentration of the sample solution, eluent volume fraction, the adsorption flow rate and the volume of the eluent as the key factors for the separation and purification of total saponins. The P values of the models established by Box-Behnken design were all below 0.05, indicating that the models had good predictive abilities. The obtained best parameters was are as follows: the mass concentration of the sample solution is 0.80 mg·mL-1, the ethanol volume fraction is 90.0%, the adsorption flow rate is 1.7 BV·h-1, and the eluent volume is 1.6 BV. Conclusion: The separation and purification method obtained by failure mode analysis combined with Box-Behnken optimization was stable and reliable, with high recovery rate and purity of saponins. This method is suitable for purifying Yixintai total saponins.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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