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Objective: To develop the determination method of the contents of 3′-hydroxy puerarin, puerarin, puerarin apioside, narirutin, naringin, hesperidin, neohesperidin, and praeruptorin A in Shensu Ganmao tablets (SGT) using high performance liquid chromatography with two-reference multi-component method (TRMC). Methods: The analyses were performed on an Agilent ZORBAX SB-C18 column (250 mm×4.6 mm, 5 μm) using the mobile phase of 0.1% phosphoric acid-acetonitrile in gradient elution mode at a flow rate of 1.0 mL·min-1. The detection wavelength was 283 nm. Results: Taking puerarin as internal reference, the RCF of 3′-hydroxy puerarin and puerarin apioside are 0.853 and 1.354. Taking naringin as internal reference, the relative correlation factors (RCF) of narirutin, hesperidin, neohesperidin, and praeruptorin A are 1.181, 1.027, 1.002, and 2.188. The RCF showed validated on different experimental conditions. The results of 8 components in SGT were in acceptable limits by using TRMC and the external standard method (ESM). Conclusion: The newly established TRMC can determine the characteristic constituents in Puerariae Lobatae Radix, Citri Reticulatae Pericarpium, Aurantii Fructus, and Peucedani Radix in SGT simultaneously, which can be used for quality control of SGT.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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