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Quality comparison research of Lactasin strain and Biofermin strain based on gene function analysis
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Bo DING1, 2, 3, 4, 5, Zhen SHEN1, 2, 3, 4, Li-hong REN1, 2, 3, 4, Xiao-jie XU1, 2, 3, 4, Sheng XING1, 2, 3, 4, Xin-yong LIU5
Chinese Journal of New Drugs | 2023, 32(6) : 643 - 656
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Chinese Journal of New Drugs | 2023, 32(6): 643-656
Quality comparison research of Lactasin strain and Biofermin strain based on gene function analysis
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Bo DING1, 2, 3, 4, 5, Zhen SHEN1, 2, 3, 4, Li-hong REN1, 2, 3, 4, Xiao-jie XU1, 2, 3, 4, Sheng XING1, 2, 3, 4, Xin-yong LIU5
Affiliations
  • 1Shan Dong Institute for Food and Drug Control, Jinan 250101, China
  • 2Shandong Food and Drug Safety Testing Engineering Technology Research Center, Jinan 250101, China
  • 3NMPA Key Laboratory for Research and Evaluation of Generic Drugs, Jinan 250101, China
  • 4Shandong Research Center of Engineering and Technology for Consistency Evaluation of Generic Drugs, Jinan 250101, China
  • 5School of Pharmaceutical Sciences Shandong University, Jinan 250012, China
Published: 2023-03-30
Outline
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Objective:

To evaluate and compare the safety and efficacy of microecological live bacteria preparations Lactasin (Lactasin strain) and Biofermin (Biofermin strain) production strains at the genome level using high-throughput sequencing and bioinformatics technology, thus to provide basis for the quality research and consistency evaluation of strains used in the production of ecological live bacteria products.

Methods:

Combined with a personalized database, DIAMOND retrieval strategy was used to find target genes and analyze their functions through sequence comparison.

Results:

The strains used for Lactasin and Biofermin production were both Enterococcus faecium. The genomes of the 2 strains had good collinearity, but a few of genes had rearrangements such as insertions, deletions, inversions, and translocations. The safety of the Biofermin strain was similar to the Lactasin strain in 6 aspects, including virulence genes, drug resistance genes, bacteriocin genes, biogenic amine genes, prophages and mobile genetic elements. In the evaluation of probiotic function of stress response genes, antimicrobial/antagonistic activity, antioxidant activity genes and adhesion genes, Biofermin strain was better than Lactasin strains.

Conclusion:

In this paper, a quality evaluation method of strains for the production of microecological live bacteria products was initially established based on specific gene function analysis, and the quality research and quality evaluation system were also improved and perfected for microecological live bacteria products.

lactasin  /  biofermin  /  Enterococcus faecium  /  bioinformatics  /  functional genes  /  probiotic screening  /  drug consistency evaluation
Bo DING, Zhen SHEN, Li-hong REN, Xiao-jie XU, Sheng XING, Xin-yong LIU. Quality comparison research of Lactasin strain and Biofermin strain based on gene function analysis[J]. Chinese Journal of New Drugs, 2023 , 32 (6) : 643 -656 .
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Year 2023 volume 32 Issue 6
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Article Info
  • Online Date：2026-03-06
  • Published：2023-03-30
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History
  • Accepted：2023-01-03
Funding
Affiliations
    1Shan Dong Institute for Food and Drug Control, Jinan 250101, China
    2Shandong Food and Drug Safety Testing Engineering Technology Research Center, Jinan 250101, China
    3NMPA Key Laboratory for Research and Evaluation of Generic Drugs, Jinan 250101, China
    4Shandong Research Center of Engineering and Technology for Consistency Evaluation of Generic Drugs, Jinan 250101, China
    5School of Pharmaceutical Sciences Shandong University, Jinan 250012, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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